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KMID : 0380019950100040435
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 4 p.435 ~ p.439
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Effect of Polyamine on Hairy Root Culture of bupleurum falcatum L.
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Abstract
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During culture the effect of polyamine on hairy root of Buplerum falcatum by infection of Agrobacterium rhizogenes was studied. The fresh and dry weight of hairy root which cultured for 3months in MS medium increased about 7-fold when spermine at 10μM, 100μM was treated. After suspension culture of B. falcatum in MS medium containing putrescine(10pM, 100μM) or spermine(10pM), the contents of endogenous polyamine (putrescine, spermidine, spermine) was higher than that of control. The /6-glucan synthetase II activity by polyamine treatment was increased: especially spermidine (100μM) and spermine(10OpM) stimulated it by about 180% and 220% respectively. These results suggest a possible role of polyamine as growth regulator in B. falcatum hairy root cultures.
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